PCR: | p21 wt | ||||
Name of Positive CTRL: | p21 wt | Annealing Temperature: | 64.0 °C | ||
Number of cycles: | 35.0 | Extension Time: | 60 s | ||
Hot Start: | HS 65 | ||||
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
IMR8294 | 424 | 100 µM | 20.0 µl for 1ml | AAG CCT TGA TTC TGA TGT GGG C | |
oIMR0845 | 423 | 100 µM | 20.0 µl for 1ml | TGA CGA AGT CAA AGT TCC ACC | |
Expected Bands: | Band Size: 872.0 | Description: wt | (text displayed in the image) | ||
More Information in PDF: | view PDF | ||||