PCR: | HIF1a-fl/wt | ||||
Name of Positive CTRL: | HIF1a-fl/wt | Annealing Temperature: | 59.0 °C | ||
Number of cycles: | 35.0 | Extension Time: | 30 s | ||
Hot Start: | Not yet | ||||
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
HIF-J HF 26 GGG | 447 | 100 µM | 10.0 µl for 1ml | TGATGTGGGTGCTGGTGTC | |
HIF-J HF27 | 448 | 100 µM | 10.0 µl for 1ml | TTGTGTTGGGGCAGTACTG | |
Expected Bands: | Band Size: 312.0 | Description: wt | (text displayed in the image) | ||
Band Size: 350.0 | Description: floxed | (text displayed in the image) | |||
More Information in PDF: | view PDF | ||||