PCR: eYFP-fl/wt
Name of Positive CTRL:eYFP-fl/wt
Annealing Temperature:58.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
EYFP-com441100 µM20.0 µl for 1mlAAA GTC GCT CTG AGT TGT TAT
EYFP-mut440100 µM10.0 µl for 1mlAAG ACC GCG AAG AGT TTG TC
EYFP-wt442100 µM10.0 µl for 1mlGGA GCG GGA GAA ATG GAT ATG
Expected Bands:Band Size: 320.0Description: 320 tg(text displayed in the image)
Band Size: 600.0Description: 600 wt(text displayed in the image)
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