PCR:	eYFP-fl/wt
Name of Positive CTRL:	eYFP-fl/wt
Annealing Temperature:	58.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	EYFP-com	441	100 µM	20.0 µl for 1ml	AAA GTC GCT CTG AGT TGT TAT
	EYFP-mut	440	100 µM	10.0 µl for 1ml	AAG ACC GCG AAG AGT TTG TC
	EYFP-wt	442	100 µM	10.0 µl for 1ml	GGA GCG GGA GAA ATG GAT ATG
Expected Bands:	Band Size: 320.0	Description: 320 tg	(text displayed in the image)
	Band Size: 600.0	Description: 600 wt	(text displayed in the image)
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