PCR: | pI-wt |
Name of Positive CTRL: | pI-wt |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 60 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| m28s rDNA 225 F | 390 | 10 µM | 10.0 µl for 1ml | CAG ACG TGG CGA CCC GCT GA |
| m28s rDNA-225 R | 276 | 10 µM | 10.0 µl for 1ml | GCC TCA CAC CGT CCA CGG GC |
| mEX1A F | 456 | 100 µM | 20.0 µl for 1ml | CTG TCC AAG TCA CCC CTA AC |
| R9 | 455 | 100 µM | 20.0 µl for 1ml | TCC ACT GTA CAC CTG AAC AG |
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Expected Bands: | Band Size: 225.0 | Description: m28s 225 | (text displayed in the image) |
| Band Size: 734.0 | Description: 734 wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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