PCR:	TNF-wt
Name of Positive CTRL:	TNF-ko
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	m28s rDNA 225 F	390	10 µM	10.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	10.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
	TNFa-comF	462	100 µM	20.0 µl for 1ml	TAG CCA GGA GGG AGA ACA GA
	TNFa-wtR	463	100 µM	20.0 µl for 1ml	AGT GCC TCT TCT GCC AGT TC
Expected Bands:	Band Size: 183.0	Description: 183 wt	(text displayed in the image)
	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
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