Name of Positive CTRL:TNF-ko
Annealing Temperature:62.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
m28s rDNA 225 F39010 µM10.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM10.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
TNFa-comF462100 µM20.0 µl for 1mlTAG CCA GGA GGG AGA ACA GA
TNFa-wtR463100 µM20.0 µl for 1mlAGT GCC TCT TCT GCC AGT TC
Expected Bands:Band Size: 183.0Description: 183 wt(text displayed in the image)
Band Size: 225.0Description: m28s 225(text displayed in the image)
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