PCR:	Siglec2-wt
Name of Positive CTRL:	Siglec2-wt
Annealing Temperature:	57.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	CD22-wtF	459	100 µM	10.0 µl for 1ml	CTC GTC TTA CCT TTT TGT TCC TCA
	CD22-wtR	460	100 µM	10.0 µl for 1ml	AGT CCA GAG ACC ATC GGC AAG
Expected Bands:	Band Size: 286.0	Description: 286 wt	(text displayed in the image)
	Band Size: 500.0	Description: 500 actin	(text displayed in the image)
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