PCR: Siglec2-wt
Name of Positive CTRL:Siglec2-wt
Annealing Temperature:57.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
CD22-wtF459100 µM10.0 µl for 1mlCTC GTC TTA CCT TTT TGT TCC TCA
CD22-wtR460100 µM10.0 µl for 1mlAGT CCA GAG ACC ATC GGC AAG
Expected Bands:Band Size: 286.0Description: 286 wt(text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
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