PCR:	OT2-TCRb
Name of Positive CTRL:	OT2-TCRb
Annealing Temperature:	60.0 °C
Number of cycles:	40.0
Extension Time:	60 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	IMRI 1825	509	100 µM	20.0 µl for 1ml	GCT GCT GCA CAG ACC TAC T
	IMRI 1826	510	100 µM	20.0 µl for 1ml	CAG CTC ACC TAA CAC GAG GA
	m28s rDNA 225 F	390	10 µM	20.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	20.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
Expected Bands:	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
	Band Size: 500.0	Description: Tcrb	(text displayed in the image)
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