PCR:	OT2-TCRa
Name of Positive CTRL:	OT2-TCRa
Annealing Temperature:	60.0 °C
Number of cycles:	40.0
Extension Time:	60 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	20.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	20.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	IMRI 1880	508	100 µM	20.0 µl for 1ml	AAA GGG AGA AAA AGC TCT CC
	IMRI 1881	507	100 µM	20.0 µl for 1ml	ACA CAG CAG GTT CTG GGT TC
Expected Bands:	Band Size: 160.0	Description: 160 Tcra	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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