PCR: | Flt3-ko/wt |
Name of Positive CTRL: | Flt3-ko/wt |
Annealing Temperature: | 65.0 °C | Number of cycles: | 50.0 |
Extension Time: | 40 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| flt3 i2-3.2 fw | 445 | 100 µM | 10.0 µl for 1ml | TCC ACG TTG TTC CCT CTA CC |
| flt3 i3-4 rev | 446 | 100 µM | 10.0 µl for 1ml | TAT GTG GGC AAT TTG GCT CT |
| flt3 neo.1 fw | 443 | 100 µM | 10.0 µl for 1ml | TGC CGC GCT GTT CTC CTC T |
| flt3 neo.2 rev | 444 | 100 µM | 10.0 µl for 1ml | AAG CGG CCA TTT TCC ACC AT |
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Expected Bands: | Band Size: 250.0 | Description: wt | (text displayed in the image) |
| Band Size: 650.0 | Description: neo | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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