PCR: HSC-Cre-tg
Name of Positive CTRL:HSC-Cre-tg
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
CreERT as450100 µM20.0 µl for 1mlAAG GCC AGG CTG TTC TTC TTA G
CreERT sense449100 µM20.0 µl for 1mlGAA CGT GCA AAA CAG GCT CTA G
m28s rDNA 225 F39010 µM15.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM15.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
Expected Bands:Band Size: 225.0Description: m28s 225(text displayed in the image)
Band Size: 733.0Description: CreERT(text displayed in the image)
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