PCR: | MacGreen |
Name of Positive CTRL: | MacGreen |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| m28s rDNA 225 F | 390 | 10 µM | 20.0 µl for 1ml | CAG ACG TGG CGA CCC GCT GA |
| m28s rDNA-225 R | 276 | 10 µM | 20.0 µl for 1ml | GCC TCA CAC CGT CCA CGG GC |
| MacGreen_fwd | 400 | 100 µM | 20.0 µl for 1ml | CTG GTC GAG CTG GAC GGC GAC G |
| MacGreen_rev | 401 | 100 µM | 20.0 µl for 1ml | CCAGAGACGGAAATCCATCGCTCG |
|
Expected Bands: | Band Size: 225.0 | Description: m28s 225 | (text displayed in the image) |
| Band Size: 670.0 | Description: 670 MG | (text displayed in the image) |
|
|
More Information in PDF: | | view PDF | |
|