PCR: PrP Ed wt
Name of Positive CTRL:PrP Ed wt
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
129Ola1271100 µM20.0 µl for 1mlATGGTGGTAGTTGGGGTCAG
129Ola2272100 µM20.0 µl for 1mlTCATCTTCACATCGGTCTCG
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Expected Bands:Band Size: 387.0Description: 387wt (text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
More Information in PDF: view PDF