PCR:	TPH wt
Name of Positive CTRL:	TPH
Annealing Temperature:	61.0 °C
Number of cycles:	40.0
Extension Time:	60 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	GTPHuni fwd	188	100 µM	20.0 µl for 1ml	ggc ttt aca gcg tct tag gg
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
	TPHEx2c rev	370	100 µM	20.0 µl for 1ml	cag cac ttt tat gag tcc tcc
Expected Bands:	Band Size: 247.0	Description: mTUBB 247	(text displayed in the image)
	Band Size: 480.0	Description: 480 TPHwt	(text displayed in the image)
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