PCR: TPH wt
Name of Positive CTRL:TPH
Annealing Temperature:61.0 °C
Number of cycles:40.0
Extension Time:60 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
GTPHuni fwd188100 µM20.0 µl for 1mlggc ttt aca gcg tct tag gg
mTUBB5-247 F277100 µM10.0 µl for 1mlCGG CCA CCA TGA GCG GCG TC
mTUBB5-247 R392100 µM10.0 µl for 1mlGTG AGG TAC CGG CCG TGG CG
TPHEx2c rev370100 µM20.0 µl for 1mlcag cac ttt tat gag tcc tcc
Expected Bands:Band Size: 247.0Description: mTUBB 247 (text displayed in the image)
Band Size: 480.0Description: 480 TPHwt (text displayed in the image)
 
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