PCR:	Ai6wt/mut
Name of Positive CTRL:	Ai6wt/mut
Annealing Temperature:	60.0 °C
Number of cycles:	40.0
Extension Time:	40 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Ai6 mut Fwd	116	100 µM	10.0 µl for 1ml	AAC CAG AAG TGG CAC CTG AC
	Ai6 mut rev	117	100 µM	10.0 µl for 1ml	GGC ATT AAA GCA GCG TAT CC
	Ai6 wt Fwd	118	100 µM	10.0 µl for 1ml	AAG GGA GCT GCA GTG GAG TA
	Ai6 wt rev	119	100 µM	10.0 µl for 1ml	CCG AAA ATC TGT GGG AAG TC
Expected Bands:	Band Size: 199.0	Description: 199mut	(text displayed in the image)
	Band Size: 297.0	Description: 297wt	(text displayed in the image)
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