PCR: Ai6wt/mut
Name of Positive CTRL:Ai6wt/mut
Annealing Temperature:60.0 °C
Number of cycles:40.0
Extension Time:40 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Ai6 mut Fwd116100 µM10.0 µl for 1mlAAC CAG AAG TGG CAC CTG AC
Ai6 mut rev117100 µM10.0 µl for 1mlGGC ATT AAA GCA GCG TAT CC
Ai6 wt Fwd118100 µM10.0 µl for 1mlAAG GGA GCT GCA GTG GAG TA
Ai6 wt rev119100 µM10.0 µl for 1mlCCG AAA ATC TGT GGG AAG TC
Expected Bands:Band Size: 199.0Description: 199mut (text displayed in the image)
Band Size: 297.0Description: 297wt (text displayed in the image)
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