PCR: Prp Ed Ko
Name of Positive CTRL:Prp Ed Ko
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
129Ola3273100 µM20.0 µl for 1mlGGATGATCTGGACGAAGAGC
129Ola4274100 µM20.0 µl for 1mlAATATCACGGGTAGCCAACG
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Expected Bands:Band Size: 220.0Description: 220 ko (text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
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