PCR:	Prp Ed Ko
Name of Positive CTRL:	Prp Ed Ko
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	129Ola3	273	100 µM	20.0 µl for 1ml	GGATGATCTGGACGAAGAGC
	129Ola4	274	100 µM	20.0 µl for 1ml	AATATCACGGGTAGCCAACG
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
Expected Bands:	Band Size: 220.0	Description: 220 ko	(text displayed in the image)
	Band Size: 500.0	Description: 500 actin	(text displayed in the image)
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