PCR:	Ltb-/-
Name of Positive CTRL:	Ltb-/+
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	LT b-1048-wt	231	100 µM	10.0 µl for 1ml	GAG ACA GTC ACA CCT GTT G
	LT b-1049-wtko	232	100 µM	20.0 µl for 1ml	CCT GTA GTC CAC CAT GTC G
	LT b-1050-ko	233	100 µM	10.0 µl for 1ml	CCT GTT CAA TGG CCG ATC C
Expected Bands:	Band Size: 120.0	Description: 120 wt	(text displayed in the image)
	Band Size: 330.0	Description: 330 ko	(text displayed in the image)
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