PCR:	GFAP-LTab
Name of Positive CTRL:	GFAP-LTab
Annealing Temperature:	56.0 °C
Number of cycles:	40.0
Extension Time:	35 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	GFAP-fwd3	178	100 µM	20.0 µl for 1ml	CAG AGC AGG TTG GAG AGG
	LT ab-LT alpha	227	100 µM	10.0 µl for 1ml	cag aga aaa cca cct ggg ag
	LT ab-LT beta	228	100 µM	10.0 µl for 1ml	gag tct ctg aga ggc tag ag
Expected Bands:	Band Size: 350.0	Description: 350GFAPLTb	(text displayed in the image)
	Band Size: 400.0	Description: 400GFAPLta	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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