PCR: | LTab |
Name of Positive CTRL: | LTab |
Annealing Temperature: | 50.0 °C | Number of cycles: | 40.0 |
Extension Time: | 35 s | Hot Start: | HS 53 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Actin fwd | 114 | 100 µM | 10.0 µl for 1ml | TGT TAC CAA CTG GGA CGA CA |
| Actin rev | 115 | 100 µM | 10.0 µl for 1ml | GAC ATG CAA GGA GTG CAA GA |
| LT ab-LT alpha | 227 | 100 µM | 10.0 µl for 1ml | cag aga aaa cca cct ggg ag |
| LT ab-LT beta | 228 | 100 µM | 10.0 µl for 1ml | gag tct ctg aga ggc tag ag |
| LT ab-prp 5' | 229 | 100 µM | 20.0 µl for 1ml | ctg agt ata ttt cag aac tg |
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Expected Bands: | Band Size: 247.0 | Description: mTUBB5-247 | (text displayed in the image) |
| Band Size: 350.0 | Description: 350 LTb | (text displayed in the image) |
| Band Size: 400.0 | Description: 400 LTa | (text displayed in the image) |
| Band Size: 500.0 | Description: 500 Actin | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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