PCR:	PDGFB ret wt
Name of Positive CTRL:	PDGFB ret wt
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 62
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	PRIMER AA4 (retwt fw)	303	100 µM	20.0 µl for 1ml	CAT GCT GCC TTG TAA TCC GTTC
	PRIMER AA5 (retwt rev)	304	100 µM	20.0 µl for 1ml	CGG CGG ATT CTC ACC GT
Expected Bands:	Band Size: 340.0	Description: 340 wt	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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