PCR: | JNK2 flox | ||||
Name of Positive CTRL: | JNK2 flox | Annealing Temperature: | 60.0 °C | ||
Number of cycles: | 35.0 | Extension Time: | 30 s | ||
Hot Start: | Not yet | ||||
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
GS.J2H3-F | 185 | 100 µM | 10.0 µl for 1ml | GTTTTGTAAAGGGAGCCGAC | |
GS.J2H3-R | 186 | 100 µM | 10.0 µl for 1ml | CCTGACTACTGAGCCTGGTTTCTC | |
Expected Bands: | Band Size: 224.0 | Description: 224 wt | (text displayed in the image) | ||
Band Size: 264.0 | Description: 264 mut | (text displayed in the image) | |||
More Information in PDF: | view PDF | ||||