PCR: | YAC |
Name of Positive CTRL: | YAC |
Annealing Temperature: | 58.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 20.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 20.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| YAC 1-pmp22 | 417 | 100 µM | 20.0 µl for 1ml | GCTACTTGGAGCCACTATCGACTACGCGAT |
| YAC 2-pmp22 | 418 | 100 µM | 20.0 µl for 1ml | GTGATAAATTAAAGTCTTGCGCCTTAAACC |
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Expected Bands: | Band Size: 228.0 | Description: 228 | (text displayed in the image) |
| Band Size: 247.0 | Description: mTUBB5-24 | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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