PCR:	Aifm mut
Name of Positive CTRL:	Aifm mut
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	40 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Aifm-1817	49	100 µM	20.0 µl for 1ml	CTA TGC CCT TCT CCA TGT AGT T
	Aimf-1819mut	51	100 µM	20.0 µl for 1ml	CCA GAA ACT GTC TCA AGG TTC C
	m28s rDNA 225 F	390	10 µM	10.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	10.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
Expected Bands:	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
	Band Size: 500.0	Description: 500 mut	(text displayed in the image)
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