PCR:	Aifm wt
Name of Positive CTRL:	Aifm wt
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	40 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Aifm-1817	49	100 µM	20.0 µl for 1ml	CTA TGC CCT TCT CCA TGT AGT T
	Aimf-1818wt	50	100 µM	20.0 µl for 1ml	AGT GTC CAG TCA AAG TAC CGG G
	mTUBB5-247 F	277	100 µM	5.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	5.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: mTUBB5-247	(text displayed in the image)
	Band Size: 520.0	Description: 520 wt	(text displayed in the image)
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