PCR: Aifm wt
Name of Positive CTRL:Aifm wt
Annealing Temperature:62.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Aifm-181749100 µM20.0 µl for 1mlCTA TGC CCT TCT CCA TGT AGT T
Aimf-1818wt50100 µM20.0 µl for 1mlAGT GTC CAG TCA AAG TAC CGG G
mTUBB5-247 F277100 µM5.0 µl for 1mlCGG CCA CCA TGA GCG GCG TC
mTUBB5-247 R392100 µM5.0 µl for 1mlGTG AGG TAC CGG CCG TGG CG
Expected Bands:Band Size: 247.0Description: mTUBB5-247(text displayed in the image)
Band Size: 520.0Description: 520 wt(text displayed in the image)
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