PCR:	CAPN4 flox
Name of Positive CTRL:	CAPN4 flox
Annealing Temperature:	64.0 °C
Number of cycles:	39.0
Extension Time:	30 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	CAPN4-fwd(1)	134	100 µM	10.0 µl for 1ml	GTC AGG CTA GAT GCC ATG TTC C
	CAPN4-rev-fl(2)	136	100 µM	10.0 µl for 1ml	GTT CAC TTG GAT CTG TCC GGT GCC
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: 247 mTUBB	(text displayed in the image)
	Band Size: 450.0	Description: 450fl	(text displayed in the image)
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