PCR: | Tg37/43 |
Name of Positive CTRL: | Tg37/43 |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 40 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| m28s rDNA 225 F | 390 | 10 µM | 20.0 µl for 1ml | CAG ACG TGG CGA CCC GCT GA |
| m28s rDNA-225 R | 276 | 10 µM | 20.0 µl for 1ml | GCC TCA CAC CGT CCA CGG GC |
| tg 37/46 fw | 349 | 100 µM | 20.0 µl for 1ml | gcgccgtgagcaggccca |
| tg 37/46 rev | 350 | 100 µM | 20.0 µl for 1ml | gtataatgtatgctatacgaagttatctcatccc |
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Expected Bands: | Band Size: 225.0 | Description: m28s rDNA | (text displayed in the image) |
| Band Size: 400.0 | Description: 400 | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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