PCR: | Cre(MCL1) |
Name of Positive CTRL: | Cre(MCL1) |
Annealing Temperature: | 59.0 °C | Number of cycles: | 40.0 |
Extension Time: | 45 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Actin fwd | 114 | 100 µM | 10.0 µl for 1ml | TGT TAC CAA CTG GGA CGA CA |
| Actin rev | 115 | 100 µM | 10.0 µl for 1ml | GAC ATG CAA GGA GTG CAA GA |
| Cre (Mcl-1) fwd | 56 | 100 µM | 10.0 µl for 1ml | GCA CTG ATT TCG ACC AGG TT |
| Cre (Mcl-1) rev | 57 | 100 µM | 10.0 µl for 1ml | CCC GGC AAA ACA GGT AGT TA |
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Expected Bands: | Band Size: 310.0 | Description: 310 Cre | (text displayed in the image) |
| Band Size: 510.0 | Description: 510 Actin | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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