PCR: | hCAST |
Name of Positive CTRL: | hCAST |
Annealing Temperature: | 58.0 °C | Number of cycles: | 35.0 |
Extension Time: | 30 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| CAST F | 137 | 100 µM | 20.0 µl for 1ml | AAGGCCATTCCAGTCAGCCA |
| CAST R | 138 | 100 µM | 20.0 µl for 1ml | AGCAACACTCTCTCCAC |
| m28s rDNA 225 F | 390 | 10 µM | 20.0 µl for 1ml | CAG ACG TGG CGA CCC GCT GA |
| m28s rDNA-225 R | 276 | 10 µM | 20.0 µl for 1ml | GCC TCA CAC CGT CCA CGG GC |
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Expected Bands: | Band Size: 225.0 | Description: m28s 225 | (text displayed in the image) |
| Band Size: 300.0 | Description: 300 | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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