PCR: Ai14
Name of Positive CTRL:Ai14
Annealing Temperature:61.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Ai14mut-fwd47100 µM10.0 µl for 1mlGGC ATT AAA GCA GCG TAT CC
Ai14mut-rev48100 µM10.0 µl for 1mlCTG TTC CTG TAC GGC ATG G
Ai14wt-fwd45100 µM20.0 µl for 1mlAAG GGA GCT GCA GTG GAG TA
Ai14wt-rev46100 µM20.0 µl for 1mlCCG AAA ATC TGT GGG AAG TC
Expected Bands:Band Size: 196.0Description: 196 mut (text displayed in the image)
Band Size: 297.0Description: 297 wt (text displayed in the image)
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