PCR:	Ai14
Name of Positive CTRL:	Ai14
Annealing Temperature:	61.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Ai14mut-fwd	47	100 µM	10.0 µl for 1ml	GGC ATT AAA GCA GCG TAT CC
	Ai14mut-rev	48	100 µM	10.0 µl for 1ml	CTG TTC CTG TAC GGC ATG G
	Ai14wt-fwd	45	100 µM	20.0 µl for 1ml	AAG GGA GCT GCA GTG GAG TA
	Ai14wt-rev	46	100 µM	20.0 µl for 1ml	CCG AAA ATC TGT GGG AAG TC
Expected Bands:	Band Size: 196.0	Description: 196 mut	(text displayed in the image)
	Band Size: 297.0	Description: 297 wt	(text displayed in the image)
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