PCR: | LTbR |
Name of Positive CTRL: | LTbR |
Annealing Temperature: | 63.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| LTbR-4 wtko | 234 | 100 µM | 20.0 µl for 1ml | CTG GTA TGG GGT TGA CAG CG |
| LTbR-7 wt | 235 | 100 µM | 10.0 µl for 1ml | TGT CAG CCG GGG ATG TCC TG |
| LTbR-HSVTK ko | 236 | 100 µM | 10.0 µl for 1ml | ATT CGC CAA TGA CAA GAC GCT GG |
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Expected Bands: | Band Size: 220.0 | Description: 220ko | (text displayed in the image) |
| Band Size: 620.0 | Description: 620wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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