Name of Positive CTRL:LTbR
Annealing Temperature:63.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
LTbR-4 wtko234100 µM20.0 µl for 1mlCTG GTA TGG GGT TGA CAG CG
LTbR-7 wt235100 µM10.0 µl for 1mlTGT CAG CCG GGG ATG TCC TG
LTbR-HSVTK ko236100 µM10.0 µl for 1mlATT CGC CAA TGA CAA GAC GCT GG
Expected Bands:Band Size: 220.0Description: 220ko (text displayed in the image)
Band Size: 620.0Description: 620wt (text displayed in the image)
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