PCR: | RipLTa |
Name of Positive CTRL: | RipLTa |
Annealing Temperature: | 60.0 °C | Number of cycles: | 40.0 |
Extension Time: | 40 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 20.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 20.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| RIP-LT a fwd | 335 | 100 µM | 20.0 µl for 1ml | att tga ggg acg ctg tgg gct c |
| RIP-LT a rev | 337 | 100 µM | 20.0 µl for 1ml | gtg tgt gtg tag cgg agc cg |
|
Expected Bands: | Band Size: 247.0 | Description: mTUBB 247 | (text displayed in the image) |
| Band Size: 450.0 | Description: 450 | (text displayed in the image) |
|
|
More Information in PDF: | | view PDF | |
|