Name of Positive CTRL:CNCE ko
Annealing Temperature:50.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
CNP-E3antisense380100 µM20.0 µl for 1mlCCCAGCCCTTTTATTACCAC
CNP-Puro3 (neo)146100 µM20.0 µl for 1mlcat agc ctg aag aac gag a
Expected Bands:Band Size: 400.0Description: 400 ko(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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