PCR: | UblfloxedDpl |
Name of Positive CTRL: | UblfloxedDpl |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 50 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Actin fwd | 114 | 100 µM | 10.0 µl for 1ml | TGT TAC CAA CTG GGA CGA CA |
| Actin rev | 115 | 100 µM | 10.0 µl for 1ml | GAC ATG CAA GGA GTG CAA GA |
| pE2 | 2 | 100 µM | 10.0 µl for 1ml | caaccgagctgaagcattctgcct |
| UBIfloxedDpl3 | 371 | 100 µM | 10.0 µl for 1ml | ctcgctggtggagcttgctatc |
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Expected Bands: | Band Size: 500.0 | Description: 500 Actin | (text displayed in the image) |
| Band Size: 618.0 | Description: 618 CD-Dpl | (text displayed in the image) |
| Band Size: 670.0 | Description: 670Prp-Dpl | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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