PCR: UblfloxedDpl
Name of Positive CTRL:UblfloxedDpl
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:50 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
pE22100 µM10.0 µl for 1mlcaaccgagctgaagcattctgcct
UBIfloxedDpl3371100 µM10.0 µl for 1mlctcgctggtggagcttgctatc
Expected Bands:Band Size: 500.0Description: 500 Actin(text displayed in the image)
Band Size: 618.0Description: 618 CD-Dpl(text displayed in the image)
Band Size: 670.0Description: 670Prp-Dpl(text displayed in the image)
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