PCR:	RAG1 wt
Name of Positive CTRL:	RAG1 wt
Annealing Temperature:	56.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	mTUBB5-247 F	277	100 µM	15.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	15.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
	RAG1-wt sense	328	100 µM	20.0 µl for 1ml	tac cct gag ctt cag ttc
	RAG1-wt antisense	329	100 µM	20.0 µl for 1ml	caa cat ctg cct tca cgt c
Expected Bands:	Band Size: 250.0	Description: 250 mTUBB	(text displayed in the image)
	Band Size: 500.0	Description: 500	(text displayed in the image)
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