PCR: RAG1 wt
Name of Positive CTRL:RAG1 wt
Annealing Temperature:56.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
mTUBB5-247 F277100 µM15.0 µl for 1mlCGG CCA CCA TGA GCG GCG TC
mTUBB5-247 R392100 µM15.0 µl for 1mlGTG AGG TAC CGG CCG TGG CG
RAG1-wt sense328100 µM20.0 µl for 1mltac cct gag ctt cag ttc
RAG1-wt antisense329100 µM20.0 µl for 1mlcaa cat ctg cct tca cgt c
Expected Bands:Band Size: 250.0Description: 250 mTUBB (text displayed in the image)
Band Size: 500.0Description: 500 (text displayed in the image)
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