PCR: RAG1 ko
Name of Positive CTRL:RAG1 ko
Annealing Temperature:56.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
m28s rDNA 225 F39010 µM15.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM15.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
RAG1-ko Neo-I326100 µM20.0 µl for 1mlctt ggg tgg aga ggc tat tc
RAG1-ko Neo-II327100 µM20.0 µl for 1mlagg tga gat gac agg aga tc
Expected Bands:Band Size: 225.0Description: m28s 225(text displayed in the image)
Band Size: 280.0Description: 280 (text displayed in the image)
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