PCR:	RAG1 ko
Name of Positive CTRL:	RAG1 ko
Annealing Temperature:	56.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	m28s rDNA 225 F	390	10 µM	15.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	15.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
	RAG1-ko Neo-I	326	100 µM	20.0 µl for 1ml	ctt ggg tgg aga ggc tat tc
	RAG1-ko Neo-II	327	100 µM	20.0 µl for 1ml	agg tga gat gac agg aga tc
Expected Bands:	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
	Band Size: 280.0	Description: 280	(text displayed in the image)
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