PCR: | PrP ko |
Name of Positive CTRL: | PrP -/- |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 40 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Prp-3'NC | 5 | 100 µM | 30.0 µl for 1ml | CCC TCC CCC AGC CTA GAC CAC GA |
| Prp-P10 | 6 | 100 µM | 20.0 µl for 1ml | GTA CCC ATA ATC AGT GGA ACA AGC CCA GC |
| Prp-P3 | 7 | 100 µM | 10.0 µl for 1ml | ATT CGC AGC GCA TCG CCT TCT ATC GCC |
|
Expected Bands: | Band Size: 380.0 | Description: 380 ko | (text displayed in the image) |
| Band Size: 550.0 | Description: 550wt | (text displayed in the image) |
|
|
More Information in PDF: | | view PDF | |
|