PCR: | PrnpInt2 |
Name of Positive CTRL: | PrnpInt2 |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 35 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 20.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 20.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| P10rev | 4 | 100 µM | 20.0 µl for 1ml | GCT GGG CTT GTT CCA CTG ATT ATG GGT AC |
| PrnpInt2-fwd | 3 | 100 µM | 20.0 µl for 1ml | ATA CTG GGC ACT GAT ACC TTG TTC CTC AT |
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Expected Bands: | Band Size: 250.0 | Description: 250 mTUBB | (text displayed in the image) |
| Band Size: 380.0 | Description: 380 | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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