PCR:	Prnd wt
Name of Positive CTRL:	Prnd wt
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	m28s rDNA 225 F	390	10 µM	20.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	20.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
	Prnd-wt-F1	82	100 µM	20.0 µl for 1ml	cgc aag ctt atg agg ggc ata aag cac agg tt
	Prnd-wt-R1	83	100 µM	20.0 µl for 1ml	cgc gga tcc tta cct ttc cag cca gaa atc gc
Expected Bands:	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
	Band Size: 400.0	Description: 400	(text displayed in the image)
More Information in PDF:		view PDF