PCR:	Prnd ko
Name of Positive CTRL:	Prnd ko
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	m28s rDNA 225 F	390	10 µM	10.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	10.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
	Prnd-ko-F2	84	100 µM	20.0 µl for 1ml	tag gtc agg cca ttt gat tcc aac
	Prnd-ko-R2	85	100 µM	20.0 µl for 1ml	tg agt ctt gga ggg atc agg agg
Expected Bands:	Band Size: 225.0	Description: m28s 225	(text displayed in the image)
	Band Size: 560.0	Description: 560	(text displayed in the image)
More Information in PDF:		view PDF