PCR:	Prn wt
Name of Positive CTRL:	Prn wt
Annealing Temperature:	56.0 °C
Number of cycles:	35.0
Extension Time:	40 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
	Prn-wt (DplBamH1) antisense	309	100 µM	20.0 µl for 1ml	cgc gga tcc tta cct ttc cag cca gaa atc gc
	Prn-wt (DplHind3ATG) sense	310	100 µM	20.0 µl for 1ml	cgc aag ctt atg agg ggc ata aag cac agg tt
Expected Bands:	Band Size: 247.0	Description: mTUBB 247	(text displayed in the image)
	Band Size: 400.0	Description: 400 Prn wt	(text displayed in the image)
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