PCR: P53 wt
Name of Positive CTRL:P53 wt
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
p53-3622100 µM10.0 µl for 1mlAGAGCAAGAATAAGTCAGAAGCCG
p53-5623100 µM10.0 µl for 1mlGTCCGCGCCATGGCCATCTA
Expected Bands:Band Size: 180.0Description: 180 wt(text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
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