PCR: | P53 wt |
Name of Positive CTRL: | P53 wt |
Annealing Temperature: | 55.0 °C | Number of cycles: | 35.0 |
Extension Time: | 30 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Actin fwd | 114 | 100 µM | 10.0 µl for 1ml | TGT TAC CAA CTG GGA CGA CA |
| Actin rev | 115 | 100 µM | 10.0 µl for 1ml | GAC ATG CAA GGA GTG CAA GA |
| p53-3 | 622 | 100 µM | 10.0 µl for 1ml | AGAGCAAGAATAAGTCAGAAGCCG |
| p53-5 | 623 | 100 µM | 10.0 µl for 1ml | GTCCGCGCCATGGCCATCTA |
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Expected Bands: | Band Size: 180.0 | Description: 180 wt | (text displayed in the image) |
| Band Size: 500.0 | Description: 500 actin | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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