Name of Positive CTRL:NSE
Annealing Temperature:62.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
m28s rDNA 225 F39010 µM10.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM10.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
NSE E1 rev279100 µM20.0 µl for 1mlgacgcagcgcccagaatcgg
NSE I'1 fwd280100 µM20.0 µl for 1mlactggtcggtgaagctgctgaggg
Expected Bands:Band Size: 225.0Description: 225 m28S (text displayed in the image)
Band Size: 390.0Description: 390 (text displayed in the image)
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