PCR: | NGPI |
Name of Positive CTRL: | NGPI |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 40 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 10.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 10.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| pE2 | 2 | 100 µM | 20.0 µl for 1ml | caaccgagctgaagcattctgcct |
| Prp-3'NC | 5 | 100 µM | 20.0 µl for 1ml | CCC TCC CCC AGC CTA GAC CAC GA |
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Expected Bands: | Band Size: 247.0 | Description: 247mTUBB | (text displayed in the image) |
| Band Size: 650.0 | Description: 650 | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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