PCR: Mfg-Tomato
Name of Positive CTRL:Mfg-Tomato
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Mfge8 E1 3 down247100 µM20.0 µl for 1mlCACGTTCGCCTCTCCTACC
mTUBB short F52710 µM15.0 µl for 1mlACCGGGCCCTCACTGTGCCTG
mTUBB short R52810 µM15.0 µl for 1mlCGTCCACGGAAGACGGCGGCA
Tom poly A369100 µM20.0 µl for 1mlCTGTTCCTGTACGGCATGG
Expected Bands:Band Size: 118.0Description: mTUBB5-118(text displayed in the image)
Band Size: 625.0Description: 625 mfg-To(text displayed in the image)
 
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