PCR: Mfg-LTb
Name of Positive CTRL:Mfg-LTb
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
LT b rev230100 µM10.0 µl for 1mlaggccagcacagccaggaca
Mfge8 E1 3 up248100 µM10.0 µl for 1mlCACTTCAGCCCTCCCTCTTC
Expected Bands:Band Size: 300.0Description: unspB6ctrl(text displayed in the image)
Band Size: 330.0Description: 330Mfg-LTb(text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
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