PCR: mfg-ERT2
Name of Positive CTRL:mfg-ERT2
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 65
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Cre102r150100 µM20.0 µl for 1mlCGC CGC ATA ACC AGT GAA AC
F1171100 µM20.0 µl for 1mlCCCACTGTGGTCTCGGAGCT
mTUBB5-247 F277100 µM10.0 µl for 1mlCGG CCA CCA TGA GCG GCG TC
mTUBB5-247 R392100 µM10.0 µl for 1mlGTG AGG TAC CGG CCG TGG CG
Expected Bands:Band Size: 247.0Description: 247 mTUBB(text displayed in the image)
Band Size: 484.0Description: 484 mfgERT(text displayed in the image)
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