PCR: | MFGE8 wt/ko |
Name of Positive CTRL: | MFGE8 wt/ko |
Annealing Temperature: | 57.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| MFG-E8 antisense | 246 | 100 µM | 40.0 µl for 1ml | GGGCATAAACTCCAGCTCAC |
| MFG-E8 ko sense | 249 | 100 µM | 20.0 µl for 1ml | CGTGGGATCATTGTTTTTCT |
| MFG-E8 wt sense | 250 | 100 µM | 20.0 µl for 1ml | GTGAACCTTCTGCGGAAGAT |
|
Expected Bands: | Band Size: 350.0 | Description: 350 ko | (text displayed in the image) |
| Band Size: 600.0 | Description: 600wt | (text displayed in the image) |
|
|
More Information in PDF: | | view PDF | |
|