Name of Positive CTRL:MAG wt
Annealing Temperature:58.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
MAG3237100 µM10.0 µl for 1mlgca ggg aat gga gac aca cg
MAG4238100 µM10.0 µl for 1mlcac cct gcc gct gtt ttg gat aat
Expected Bands:Band Size: 250.0Description: 250 wt(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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