PCR:	Lt a ko
Name of Positive CTRL:	Lt a ko
Annealing Temperature:	55.0 °C
Number of cycles:	35.0
Extension Time:	35 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	LT a-158 ko	220	100 µM	10.0 µl for 1ml	ctg aat gaa ctg cag gac ga
	LT a-159 ko	221	100 µM	10.0 µl for 1ml	ata ctt tct cgg cag gag ca
	LT a-168 wt	222	100 µM	10.0 µl for 1ml	tcc tct cca ctc tct tct gtc c
	LT a-169 wt	226	100 µM	10.0 µl for 1ml	ggg atg gtt ggt agg gag at
Expected Bands:	Band Size: 180.0	Description: 180 ko	(text displayed in the image)
	Band Size: 280.0	Description: 280 wt	(text displayed in the image)
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