PCR:	LT a
Name of Positive CTRL:	LT a
Annealing Temperature:	50.0 °C
Number of cycles:	40.0
Extension Time:	35 s
Hot Start:	HS 53
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	5.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	5.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	LT ab-LT alpha	227	100 µM	20.0 µl for 1ml	cag aga aaa cca cct ggg ag
	LT ab-prp 5'	229	100 µM	20.0 µl for 1ml	ctg agt ata ttt cag aac tg
Expected Bands:	Band Size: 400.0	Description: 400	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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