PCR:	Cg
Name of Positive CTRL:	Cg
Annealing Temperature:	55.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Cg ko rev-pNeo	142	100 µM	10.0 µl for 1ml	cct gcg tgc aat cca tct tgt tca at
	Cg wt rev-P7	379	100 µM	10.0 µl for 1ml	gat cca gat tgc caa ggt gag tag
	Cg wtko fwd-P5	37	100 µM	20.0 µl for 1ml	ctg ctc aga atg cct cca att cc
Expected Bands:	Band Size: 400.0	Description: 400mut	(text displayed in the image)
	Band Size: 650.0	Description: 650 wt	(text displayed in the image)
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