Name of Positive CTRL:Cg
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Cg ko rev-pNeo142100 µM10.0 µl for 1mlcct gcg tgc aat cca tct tgt tca at
Cg wt rev-P7379100 µM10.0 µl for 1mlgat cca gat tgc caa ggt gag tag
Cg wtko fwd-P537100 µM20.0 µl for 1mlctg ctc aga atg cct cca att cc
Expected Bands:Band Size: 400.0Description: 400mut (text displayed in the image)
Band Size: 650.0Description: 650 wt (text displayed in the image)
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